microRNAs quantitative assay with Splinted Ligation

Do you still use Northern Blot to quantitate microRNAs expression? Here I recommend Splinted Ligation Assay[1,2], though this method is an old story (published in 2007).   Detection of miRNAs using splinted ligation. Schematic depiction of the assay process. As described in the text, the assay involves: (1) Labeling of the ligation oligonucleotide; (2) concurrent … Read more

DNA Nanotechnology Kills Cancer

Here I recommend you a wonderful bio-app, named: caDNAno. The open-source software, caDNAno,  will enables you  design  three-dimensional DNA origami nanostructures. You can fold your DNA in any shapes. One of the features is no programming required, thus even our kids can give it a try, and perhaps can get a Science paper published [1]. What will … Read more

Computer Program Enrich Understandings to RNA motifs

DNA carries and passes the genetic information with nucleotides sequences, but in some virus RNA does the job instead of DNA.  In addition, the 3D structure of RNA, RNA motif, shows more various roles in cellular functions. It means that only with sequence information we cannot fully understand the RNA roles and functions. Detecting and … Read more

Time To Question Bio-Engineering [Question Mark]

I came across this bio-ethics video when I re-watch Craig Venter’s three speeches in TED. This thoughts-provoking video is also available on its official TED page  just in case you don’t have the access to YouTube. The contents of the speaker mainly contains three parts: Typical transgenic technology; Computer Controls Brains; Synthetic Life, or synthetic biology. … Read more